The PCSK9(D374Y) [Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The kit comes in a convenient 96-well format and contains enough biotin-labeled mutant protein PCSK9(D374Y), purified LDLR ectodomain, and HRP-conjugated streptavidin for 100 reactions. Moreover, two pre-formulated assay buffers are supplied to validate PCSK9-LDLR binding in either neutral or acidic binding conditions.

This assay takes advantage of the high sensitivity of detection of biotin-labeled PCSK9(D374Y) by streptavidin-HRP. First, a 96-well plate is coated with LDLR ectodomain. PCSK9(D374Y) is then incubated with LDLR. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which is measured using a chemiluminescence reader.